PET and the multitracer concept in the study of neurodegenerative diseases / PET e o conceito de multitraçador no estudo de doenças degenerativas

ABSTRACT The complexity of the pathological reactions of the brain to an aggression caused by an internal or external noxa represents a challenge for molecular imaging. Positron emission tomography (PET) can indicate in vivo,anatomopathological changes involved in the development of different clinical symptoms in patients with neurodegenerative disorders. PET and the multitracer concept can provide information from different systems in the brain tissue building an image of the whole disease. We present here the combination of 18F-flourodeoxyglucose (FDG) and N-[11C-methyl]-L-deuterodeprenyl (DED), FDG and N-[11C-methyl] 2-(4'-methylaminophenyl)-6-hydroxybenzothiazole (PIB), PIB and L-[11C]-3'4-Dihydrophenylalanine (DOPA) and finally PIB and [15O]H2O.

RESUMO A complexidade das reações patológicas do cérebro à agressões causadas por noxa interna ou externa representa um desafio para a imagem molecular. Tomografia por emissão de positron (PET) pode indicar, in vivo, alterações anatomopatológicas envolvidas no desenvolvimento de diferentes sintomas clínicos em pacientes com desordens neurodegenerativas. PET e o conceito de multitraçador pode fornecer informações de diferentes sistemas no tecido cerebral, construindo assim uma imagem da doença como um todo. Nós apresentamos neste artigo a combinação de 18F-flourodeoxyglucose (FDG) e N-[11C-methyl]-L-deuterodeprenyl (DED), FDG e N-[11C-methyl] 2-(4'-methylaminophenyl)-6-hydroxybenzothiazole (PIB), PIB e L-[11C]-3'4-Dihydrophenylalanine (DOPA) e finalmente, PIB e [15O]H2O .

Conservación del ombusillo, planta amenazada de la provincia de Buenos Aires, Argentina / Conserving ombusillo, an endangered plant from the province of Buenos Aires, Argentina

Phytolacca tetramera Hauman "ombusillo", es una especie vegetal endémica del SE de la Provincia de Buenos Aires, Argentina, que se halla en peligro crítico de extinción. Su principal factor de amenaza es la reducción del hábitat por acción antrópica. Esta especie presenta principios activos fungicidas y, posiblemente, dada su afinidad con otras especies del mismo género, presente compuestos antivirales, antitumorales, bactericidas e insecticidas. Se realizaron ensayos de macropropagación con distintas concentraciones de reguladores de crecimiento de tipo auxínicos que muestran claramente un enraizamiento óptimo correspondiente a segmentos de ejes aéreos vegetales “estacas” sometidas a 300 ppm de ácido indol butírico y a segmentos de tallos subterráneos sin aplicación de hormonas. Así mismo, se realizaron ensayos de germinación, en condiciones de luz y de oscuridad, comprobándose que las semillas presentan fotoblastismo positivo con un porcentaje de germinación del 65%, el cual disminuye enormemente luego del año de cosecha.

Phytolacca tetramera Hauman "ombusillo" is an endemic plant species which is in critical danger of becoming extinct; it comes from the south-east of the province of Buenos Aires. The main factor threatening this species is the reduction of its natural environment by antropic action.This species has antifungal properties and, due to its relationship with other species from the same genus, it could also have antiviral, antitumour, antibacterial and insecticidal compounds. Macropropagation experiments were carried out using different concentrations of auxinic growth regulators. Segements of aerial axis “stakes” treated with 300 ppm of indol-butiric acid and segments of underground stems without hormonal treatment provided optimum rooting. Germination experiments in dark and light conditions were also carried out, finding that seeds showed positive photoblastisme with a 65% germination rate which declined considerably after the crop had been harvested.